About

The CRISPR-Cas Defense Mechanism program delves into the revolutionary gene-editing technology that has transformed scientific research and biotechnology. Over the course of one month, students will learn about different CRISPR-Cas systems, their discovery, and how they can be harnessed for genome editing across various organisms. The curriculum is designed to cover both theoretical concepts and practical applications, including lab simulations and case studies of CRISPR in therapeutic settings and agriculture.

Aim

This program aims to provide an in-depth understanding of the CRISPR-Cas systems, highlighting their roles in microbial immunity and biotechnological applications. Participants will explore the molecular mechanisms, ethical implications, and the latest advancements in CRISPR technology to prepare for careers in genetic engineering and biomedicine.

Objectives

• Understand the fundamentals of CRISPR-Cas systems and their biological functions.
• Gain hands-on experience with CRISPR design and applications in lab simulations.
• Explore the ethical and regulatory frameworks governing gene editing.
• Analyze case studies on the impact of CRISPR technologies in medicine and agriculture.
• Prepare for advanced studies or careers in genetic editing technologies.

Curriculum

1. Week 1: Introduction to Genome Editing

   • Historical perspective of genome editing.
   • Overview of genome editing technologies.
   • Discovery and development of CRISPR-Cas systems.

2. Week 2: Molecular Mechanisms of CRISPR-Cas

   • Structure and function of CRISPR-Cas components.
   • Mechanism of CRISPR-Cas action.
   • Types of CRISPR-Cas systems (Class 1, Class 2).

3. Week 3: CRISPR-Cas in Prokaryotes

   • Adaptive immunity in bacteria and archaea.
   • Natural functions and diversity of CRISPR-Cas systems.
   • Biogenesis and interference phases.

4. Week 4: Guide RNA Design and Optimization

   • Principles of guide RNA design.
   • Bioinformatics tools for target site selection.
   • Strategies to enhance specificity and efficiency.

Outcomes

• Proficiency in CRISPR-Cas technology applications.
• Understanding of molecular techniques in genetic engineering.
• Ability to address ethical considerations in biotech.
• Readiness for advanced research or professional roles.
• Skills in interdisciplinary collaboration and innovation.